What are the performance factors that affect large flanges?
In the production of large flanges, there are many factors affecting the performance of large flanges. Here are a few common factors.
The first is the annealing temperature (the annealing temperature is the temperature parameter when the primer and the template are combined. When 50% of the primers and complementary sequences are expressed as double-stranded DNA molecules, it is an important factor affecting PCR specificity. Under ideal conditions The annealing temperature is low enough to ensure that the primers are effectively annealed to the desired sequence and at the same time high enough to reduce non-specific binding. Reasonable annealing temperatures range from 55 ° C to 70 ° C. Annealing temperatures are generally set at 5 ° C lower than the Tm of the primers. Whether to reach the specified temperature, the large flange treatment is generally a solution heat treatment, which is commonly called "annealing", the temperature range is 1040 ~ 1120 ° C (Japanese standard). You can also pass When the furnace observation hole is observed, the large flange pipe fittings in the annealing zone should be in an incandescent state, but no softening sagging occurs.
Followed by the sealing of the furnace body (the outer shell is welded with steel plate and section steel, the trolley is welded by steel and steel plate, the trolley passes the soft contact with the furnace lining and the sand sealing mechanism to reduce heat radiation and convection loss, and effectively ensure the sealing of the furnace body. ), the bright annealing furnace should be closed and isolated from the outside air; using hydrogen as the shielding gas, only one exhaust port is open (used to ignite the discharged hydrogen). The method of inspection can be applied to the gaps of the joints of the annealing furnace with soapy water to see if the gas is running. The most easy place to run the gas is the place where the annealing furnace enters the pipe and the place where the pipe is discharged. The sealing ring in this place is particularly prone to wear. Always check frequently to change.
Again, the protective gas pressure, in order to prevent the occurrence of micro-leakage, the furnace protective gas should maintain a certain positive pressure, if it is hydrogen protective gas, generally requires more than 20kBar. Annealing atmosphere: Generally, pure hydrogen is used as the annealing atmosphere, and the purity of the atmosphere is preferably 99.99% or more. If another part of the atmosphere is an inert gas, the purity can be lower, but it must not contain too much oxygen or water vapor.